Triticum turgidum var. durum Bacterial artificial chromosome (BAC) library
1. Dept. Agronomy
and Range Science, Wheat Genomic Center,
2. Current address: Dip. Biologia e Chimica Agroforestale e Ambientale, Universita' degli Studi di Bari, Italia.
3. Current address: Institute National de la Recherche Agronomique,
5 Current addresse: The Institute of Evolution,
Publication: Cenci, A., N. Chantret, K. Xy, Y. Gu, O.D. Anderson, T. Fahima, A. Distelfeld, and J. Dubcovsky. 2003. A half million clones Bacterial Artificial Chromosome (BAC) library of durum wheat. Theor. Appl. Genet. 107:931-939.
Funding: This project was supported by USDA-BARD grant US-3224-01R, by INRA-France and by USDA-Albany.
· Material Transfer Agreement: To request clones or filters please print, sign and send to
& Range Science,
· Plant material: cultivated tetraploid wheat Triticum turgidum var. durum, cultivar Langdon with a 30 cM homozygous insertion of T. turgidum var. dicoccoides accession FA15-3 from chromosome 6BS carrying a gene for high grain protein content.
· High molecular weight DNA: nuclei extracted from leaves.
· Cloning vector: pIndigoBac536
· Library characteristics:
· 516,096 BAC clones picked in 1,344 384-well plates. Average insert size 130 kb
· High density filters production: 28 sets of high-density filters completed
· Empty clones <1%
· Chloroplast clones 0.34%
· Present library coverage (based on estimated DNA content of 13,000 Mbp/1C): 5X