Triticum turgidum var. durum Bacterial artificial chromosome (BAC) library

A. Cenci1,2, N. Chantret1,3, K. Xy4, Y. Gu4, O.D. Anderson4, T. Fahima1,5, A. Distelfeld1,5and J. Dubcovsky.1

1. Dept. Agronomy and Range Science, Wheat Genomic Center, University of California, Davis, Ca, USA.
2. Current address: Dip. Biologia e Chimica Agroforestale e Ambientale, Universita' degli Studi di Bari, Italia.
3. Current address: Institute National de la Recherche Agronomique, Rennes, France.
4. USDA Western Reg. Research Center, Wheat Genomic CenterAlbany, Ca, USA.
5 Current addresse: The Institute of Evolution, University of Haifa, Mt. Carmel, Haifa. Israel

Publication: Cenci, A., N. Chantret, K. Xy, Y. Gu, O.D. Anderson, T. Fahima, A. Distelfeld, and J. Dubcovsky. 2003. A half million clones Bacterial Artificial Chromosome (BAC) library of durum wheat. Theor. Appl. Genet. 107:931-939.

Funding: This project was supported by USDA-BARD grant US-3224-01R, by INRA-France and by USDA-Albany.

        Conditions and addresses to request clones and filters

        Material Transfer Agreement: To request clones or filters please print, sign and send to

                        J. Dubcovsky,

                        Agronomy & Range Science, University of California

                        One Shields Avenue, Davis, 95616 CA, USA

      Plant material: cultivated tetraploid wheat Triticum turgidum var. durum, cultivar Langdon with a 30 cM homozygous insertion of T. turgidum var. dicoccoides accession FA15-3 from chromosome 6BS carrying a gene for high grain protein content.

      High molecular weight DNA: nuclei extracted from leaves.

      Cloning vector: pIndigoBac536

      Library characteristics:

        516,096 BAC clones picked in 1,344 384-well plates. Average insert size 130 kb

        High density filters production: 28 sets of high-density filters completed

        Empty clones <1%

        Chloroplast clones 0.34%

        Present library coverage (based on estimated DNA content of 13,000 Mbp/1C): 5X