Virgilio C. Andaya, Ph.D. Plant Breeder

E-mail: vcandaya@crrf.org

RESEARCH INTEREST

Integration of molecular marker technology and genomics tools with conventional breeding methods in the development of improved crops with higher yield, better quality, increased economic and nutritive value, and resistance to biotic and abiotic stresses

 RESEARCH EXPERIENCE

 o       As a rice breeder in a national program, and part of a rice varietal improvement team, developed, evaluated and tested rice lines leading to the   release of rice varieties for upland and rainfed rice culture

o       Provided technical leadership in establishing, preparing, implementing, coordinating, managing, and reviewing rice breeding projects

o       Involved with collaborative projects dealing with genetics of important agronomic traits, pyramiding resistance genes using molecular markers, shuttle breeding, genotype x environment interaction analyses using regression, AMMI and pattern analysis, and crop modeling

o       Streamlined the use of molecular markers that lead to more efficient, faster, and high-throughput genotyping and developed procedures for efficient screening of rice lines for cold tolerance using growth chamber and greenhouse facilities

o       Conducted studies on genetics of cold tolerance in rice and developed a large population suited for quantitative trait loci analysis of rice traits, and designed molecular markers for marker-assisted selection, backcrossing and gene pyramiding

o       Identified and fine-mapped major genes and QTL controlling tolerance to low temperature stress of M202 at seedling and booting stages that are useful for California and screened diverse germplasm for association mapping  

TECHNICAL SKILLS

o       Experienced in conventional selection techniques and non-traditional breeding methods, field plot techniques and experimental designs

o       Proficient in the development of high-density genetic maps, and development of molecular markers for high-resolution mapping.

o       Proficient in high-throughput plant DNA extraction and genotyping procedures, PCR–based techniques including multiplexing, application of molecular marker systems especially microsatellite and ORF-based markers for marker-assisted selection/backcrossing, sequence analysis and identification of candidate genes underlying QTL

o       Proficient in the operation of DNA sequencers (ABI 377, CEQ 8000), liquid-handling robots, PCR machines, and other molecular lab equipment

o       Proficient with database applications, spreadsheet softwares, and statistics softwares in both Mac and PC platforms such as Excel, Word, PowerPoint, Mapmaker, QGene, Plabqtl, Mapqtl, Genescan, Genotyper, Systat, Statview, Geneflow, Agrobase and various Web-based databases and search tools.

o       Experienced in the development of assays and screening procedures to phenotype plant traits  specifically cold tolerance related attributes

AWARDS RECEIVED

1991-1993       Philippine Rice Research Institute Scholarship Grant

1992                Pagasa Award, Philippine Civil Service Commission

1994                Scholastic Achievement Award, PhilRice

1995                Best Masters Thesis Award, UPLB

1996-2001       Ph.D. Fellowship, Rockefeller Foundation Inc

2004                Certificate of Merit, USDA-ARS

2005-2007       USDA Research Associateship

MEMBERSHIP TO PROFESSIONAL ORGANIZATIONS

American Society of Agronomy

Crop Science Society of America

Crop Science Society of the Philippines

Phi Sigma Society, Gamma Delta Chapter, The Honor Society for the Biological Sciences, UCD

LIST OF PUBLICATIONS

Andaya VC, EL Javier and HE Hernandez. 1995. Variability and estimation of heterosis in second cycle anther culture-derived doubled haploid rice lines (Oryza sativa L. cv. IR43). Phil. J. Crop Sci. 20(1):21-27

Andaya CB, LS Sebastian, C Leaño, and VC Andaya. 1998.  Variability within doubled haploid lines from rice varieties, RPKN2 and BW 306-8. Acta Horticulturae.  Leuven Belgium : International Society for Horticultural Science. pp 293-300

Andaya VC and DJ Mackill. 2003. QTLs conferring cold tolerance at the booting stage of rice using recombinant inbred lines from a japonica x indica cross. Theor. Appl. Genet. 106:1084-1090

Andaya VC and DJ Mackill. 2003. Mapping of QTLs associated with cold tolerance during the vegetative stage in rice. J. Expt. Bot. 54:2579-2585

Andaya VC and TH Tai. 2006. Fine mapping of the qCTS12 locus, a major QTL for seedling cold tolerance in rice. Theor. Appl. Genet. 113: 467-475

Andaya VC, DJ Mackill and TH Tai. 2006. Release of M-202/IR50 (MI) Recombinant Inbred Mapping Population.  USDA-ARS, Davis , CA , U.S.A

 Andaya VC and TH Tai. 2007. Fine mapping of the qCTS4 locus associated with seedling cold tolerance in rice (Oryza sativa L.)  Mol. Breeding (10.1007/s11032-007-9096-8)

LIST OF PRESENTATIONS

Andaya VC, EL Javier and HE Hernandez. Variability in second cycle anther culture- derived doubled haploid rice lines (Oryza sativa L. cv. IR43) I. Nature of variability and estimation of heterosis. Paper presented during the FCSSP 11th Annual Scientific Conference held at Silliman University , Dumaguete City , Philippines on 22-29 April 1995.

Andaya VC, D Lalican. AMMI and pattern analysis of the 22nd International Irrigated Rice Yield Nursery. Paper presented during the International Workshop on the Analysis of Genotype by Environment Interaction at IRRI, Philippines on October 4-6, 1995

Andaya VC, D Lalican. Genotype by Environment Interaction Analysis of Results from Multilocation Trials in Rice. Paper presented during the International Network for Genetic Evaluation in Rice (INGER) Advisory Committee meeting at PhilRice , Philippines on 20 November 1995

Caldo, RA, VC Andaya, LS Sta Ines, AM Galvez, LS Sebastian and HC Dela Cruz. New Rice Varieties for Adverse Environments. Poster presented during the 8th Regional Symposium on Research and Development in Tarlac , Philippines on July 31- August 1, 1997 (garnered the BEST POSTER Award)

Andaya VC, DV Bac and DJ Mackill. Locating QTLs for Cold Tolerance at Booting Stage in Rice (Oryza sativa L.).  I. Tolerance at two temperature regimes. Poster presented during the 27^th RTWG meeting at the Nugget Hotel, Reno , Nevada , U.S.A. on March 1-4, 1998 .

Andaya VC and DJ Mackill.  Molecular mapping of rice cold tolerance using an indica-japonica crosses. Poster presented during the 2nd Temperate Rice Conference held at Holiday Inn Capitol Plaza , Sacramento , CA , U.S.A. on June 13-17, 1999 .

Andaya VC, X-M Lei and DJ Mackill. Studies on high and low temperature tolerance in rice. Poster presented during the field day at the California Rice Experiment Station, Biggs , CA , U.S.A. on August 25, 1999 .

Tai, TH, PM Colowit, VC Andaya and CB Andaya. Rice genetics and germplasm enhancement for temperate environment. Poster presented during the field day at the California Rice Experiment Station, Biggs , CA , U.S.A. in August, 2003

Tai, TH, PM Colowit, VC Andaya, CB Andaya, L Snyder and DV Aragones. USDA-ARS temperate rice project. Poster presented during the field day at the California Rice Experiment Station, Biggs , CA , U.S.A. in August, 2003

Andaya VC, DJ Mackill and TH Tai. Enhancing cold tolerance of rice for temperate environments. Poster presented during the 30^th RTWG in New Orleans , LA , U.S.A. on February 29 - March 3, 2004 .

Andaya VC and TH Tai. Linkage disequilibrium and association mapping of traits for temperate adaptation in rice. Poster presented during the field day at the California Rice Experiment Station, Biggs , CA , U.S.A. in August, 2005

Andaya VC and TH Tai. Fine mapping a major QTL for seedling cold tolerance in rice. Poster presented during the Plant & Animal Genome XIV Conference in San Diego , CA , U.S.A. on January 14-18, 2006

Andaya VC and TH Tai. Genetic Analysis of Cold Tolerance using Structured and Unstructured Populations. Poster presented during the 31^st Rice Technical Working Group Meeting in Woodlands, Texas , U.S.A. on February 26- March 1, 2006 .

Andaya VC and TH Tai. Genetic mapping of the qCTS12 and qCTS4 loci controlling M202 rice seedling response to low temperatures. Poster presented during the field day at the California Rice Experiment Station, Biggs , CA , U.S.A. on August 30, 2006 .

Andaya VC, Colowit, P., Jodari, F., Oster, J., Jiang, J., Johnson, C., Kim, S., Roughton, I. , McKenzie, K., and T. Tai. Application of DNA markers to the Rice Experiment Station Breeding Programs. Poster presented during the field day at the California Rice Experiment Station, Biggs , CA , U.S.A. on August 30, 2006 .

Andaya VC and TH Tai. 2006. Fine Mapping of Major Seedling Cold Tolerance QTL in Rice. Oral presentation during the ASA-CSSA-SSAS 2006 Annual Meetings, Indianapolis , IN on November 12-16, 2006 .

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